Generator

Part:BBa_K2152006:Design

Designed by: MingZhe Chen   Group: iGEM16_ShanghaiTechChina_B   (2016-10-13)


T7 promoter + RBS + killerred


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 238


Design Notes

The point mutation is taken to avoid the XbaI error cut


Source

killer red is from the plate 1 of 2016 distribution plates. T7 and RBS is from the pet28a vector.

References